Human Lymphotactin(XCL1) Recombinant

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Encompassing Amino Acids: 23-114

Amino Acid Sequence: GSEVSDKR TCVSLTTQRL PVSRIKTYTI TEGSLRAVIFITKRGLKVCA DPQATWVRDV VRSMDRKSNT RNNMIQTKPT GTQQSTNTAV TLTG

Background: XCL1, also known as lymphotactin or ATAC, is the sole member of the C subgroup of chemokines, and is produced mainly by activated CD8 T cells (and to a lesser extent by NK and mast cells). The chemotactic activity of XCL1 is restricted to lymphocytes and has no effect on monocytes.

Biological Activity: Activity was determined by the ability to induce chemotaxis of human T-cells at concentrations ranging between 50-100 ng/ml.

Description: Recombinant Lymphotactin is a disulfide-linked homodimeric protein consisting of 93 amino acid residues, and migrates as an approximately 10 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human lymphotactin (XCL1) mature chain was expressed in E. coli.

Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Format: lyophilized protein

Formulation: Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

Genbank: P47992

Purity: ≥97% by SDS-PAGE and HPLC

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Uniprot: P47992

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Hedrick JA, Zlotnik A. Clin Immunol Immunopathol. 1998 Jun,87(3):218-22.
2. Volkman BF, et al. Methods Enzymol. 2009, 461:51-70.

• Artikelnummer: BPS90210-A
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90210-A
• Verpackungseinheit: 5 µg
• Mengeneinheit: STK