Human NK Cell Isolation Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Isolate NK cells by depleting other immune cells from a mixed population such as PBMCs.Isolated NK cells may be used for downstream applications such as NK cell activation, ADCC (antibody-dependent cell cytotoxicity) assays, genomic analysis, expression assays, protein isolation, and flow cytometry.

Background: NK (natural killer) cells are part of the innate immune system. They function in a histocompatibility complex-independent mode and derive from the hematopoietic lineage. In PBMCs (human peripheral blood mononuclear cells) derived from healthy individuals, 5-20% of the cells are NK cells. They are the first line of defense against cancer. Expression of marker CD56 correlates with NK cell functionality: the CD56^bright subset accounts for about 5% of the population and is less cytotoxic than the CD56^dim subset.  Cytotoxicity can happen by the release of perforin and granzyme, while activation by KARs (killer activating receptors) leads to release of Fas Ligand, TRAIL (TNF-related apoptosis-inducing ligand) and TNFα (tumor necrosis factor-alpha). In a suppressive tumor microenvironment, NK cells can become inhibited and unable to fight cancer cells. Several clinical trials have focused on using ex vivo generated NK cells alone or in combination with other approaches. NK cells can be generated ex vivo from peripheral blood, umbilical cord blood, iPS cells or immortalized NK cell lines. The ability to generate a high enough number of pure cells for human dosage often requires the use of growth factors such as IL-2 (interleukin 2) or IL-15, and feeder cells. The use of NK cells or CAR (chimeric antigen receptor)-NK cells is an expanding area holding great promise in cancer therapy. NK cells are important immune cells that have a variety of functions, inducing the lysis of tumors and virally infected cells, controlling microbial infections, and regulation of T and B cell-mediated immunity.

Description: The Human NK Cell Isolation Kit is designed to magnetically separate NK cells from a complex immune cell population. This kit is optimized for the isolation of CD56⁺CD3^- cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with a mix of antibodies followed by conjugation to magnetic beads. They are then placed on a magnet for quick and easy separation. When placed on the magnet, non-NK cells will be immobilized along the side of the tube while NK cells will remain in suspension and can be easily removed for downstream applications.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: This kit is provided with enough reagents and materials for isolation of NK cells from up to 1 x 10⁸ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS82287
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82287
• Verpackungseinheit: 1 x 10⁸
• Mengeneinheit: STK