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Human Tissue Plasminogen Activator
Human Tissue Plasminogen Activator
Artikelnummer
BPS90248-B
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 36-562
Amino Acid Sequence: SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
Background: Tissue plasminogen Activator (abbreviated tPA or PLAT) is a protein involved in the breakdown of blood clots. Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells lining the blood vessels. It catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.
Biological Activity: The activity was determined to be 6 x 10^5 IU/mg.
Description: Recombinant tPA is a disulfide-linked monomer protein consisting 527 amino acid residue subunits. and migrates due to glycosylation as an approximately 60kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Tissue Plasminogen Activator mature chain was expressed in CHO cells.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered 1 mg/ml solution containing 0.2 M L-arginine, 0.1 M phosphoric acid.
Genbank: P00750
Purity: ≥98% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P00750
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ortolano S, Spuch C. Recent Pat Endocr Metab Immune Drug Discov. 2013 Jan,7(1):65-76.
2. Eissa A, et al. J Clin Pharm Ther. 2012 Dec,37(6):620-9.
3. Adibhatla RM, Hatcher JF. CNS Neurol Disord Drug Targets. 2008 Jun,7(3):243-53.
Encompassing Amino Acids: 36-562
Amino Acid Sequence: SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP
Background: Tissue plasminogen Activator (abbreviated tPA or PLAT) is a protein involved in the breakdown of blood clots. Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells lining the blood vessels. It catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.
Biological Activity: The activity was determined to be 6 x 10^5 IU/mg.
Description: Recombinant tPA is a disulfide-linked monomer protein consisting 527 amino acid residue subunits. and migrates due to glycosylation as an approximately 60kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Tissue Plasminogen Activator mature chain was expressed in CHO cells.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered 1 mg/ml solution containing 0.2 M L-arginine, 0.1 M phosphoric acid.
Genbank: P00750
Purity: ≥98% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P00750
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ortolano S, Spuch C. Recent Pat Endocr Metab Immune Drug Discov. 2013 Jan,7(1):65-76.
2. Eissa A, et al. J Clin Pharm Ther. 2012 Dec,37(6):620-9.
3. Adibhatla RM, Hatcher JF. CNS Neurol Disord Drug Targets. 2008 Jun,7(3):243-53.
| Artikelnummer | BPS90248-B |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90248-B |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Wirt | Hamster |
| Produktinformation (PDF) |
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| MSDS (PDF) |
|
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