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IDH1, FLAG-tag Recombinant
IDH1, FLAG-tag Recombinant
Artikelnummer
BPS71075-1
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was conductedin 50 mM Tris (pH 7.4), 10 mM MgCl2, 200µM DL-isocitrate, and 200 µM NADP.Incubate for 30 minutes at roomtemperature. Read at Abs340 using TecanInfinite 1000 (5 flash/10 ms settle time).
Description: Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length [a.a. 1-414(end)] with C-terminal FLAG-tag, MW= 47.6 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.
Genbank: NM_005896
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: O75874
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97.
2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43.
3. Fu, Y., et al. (2010). Biochem. Biophys. Res. Commun. 397(2):127-30.
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was conductedin 50 mM Tris (pH 7.4), 10 mM MgCl2, 200µM DL-isocitrate, and 200 µM NADP.Incubate for 30 minutes at roomtemperature. Read at Abs340 using TecanInfinite 1000 (5 flash/10 ms settle time).
Description: Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length [a.a. 1-414(end)] with C-terminal FLAG-tag, MW= 47.6 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.
Genbank: NM_005896
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: O75874
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97.
2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43.
3. Fu, Y., et al. (2010). Biochem. Biophys. Res. Commun. 397(2):127-30.
| Artikelnummer | BPS71075-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 71075-1 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
