IL-15 Responsive Luciferase Reporter Cell Line

IL-15 Responsive Luciferase Reporter Cell Line
Artikelnummer
BPS78402
Verpackungseinheit
2 vials
Hersteller
BPS Bioscience

Verfügbarkeit: wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€

Applications: Characterize recombinant IL-15, IL-15 analogs or other agonists of IL-15RScreen Janus kinase or IL15R inhibitors in a cell-based assay

Background: IL-15 is a proinflammatory cytokine mainly produced in dendric cells (DC) and monocytes. It remains associated with IL-15Rα on the surface of DC and monocytes, and binds to the CD122/CD132 heterodimeric receptor present at the surface of various target immune cells, including CD8+ memory T cells, resulting in their proliferation and differentiation. Although there is no indication that unassociated IL-15 exists in vivo, soluble recombinant IL-15 and its analogs have been examined for their therapeutic potential.

Description: This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity.

Host Cell Line: Jurkat

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1
Mehr Informationen
Artikelnummer BPS78402
Hersteller BPS Bioscience
Hersteller Artikelnummer 78402
Verpackungseinheit 2 vials
Mengeneinheit PAK
Wirt Human
Produktinformation (PDF) Download
MSDS (PDF)
×