IL2 Receptor alpha antibody (BC96) (APC)

Clone Name: BC96

Light Chain: Kappa

Product Description: The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells. The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Application Note: FACS: 0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 31

Form: Liquid

Buffer (with preservative): 10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin, 0.09% Sodium azide.

Concentration: 0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot ID: P01589

Purification: Purified by affinity chromatography

Conjugation: APC

Full Name: interleukin 2 receptor subunit alpha

• Artikelnummer: GTX01471-07-100
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01471-07-100
• Green Labware: Nein
• Verpackungseinheit: 100 test
• Mengeneinheit: PAK
• Reaktivität: Human, Primate, Cynomolgus Monkey (Primate), Rhesus Monkey (Primate)
• Klonalität: Monoclonal
• Methode: Flow Cytometry
• Isotyp: IgG1
• Human Gene ID: 3559
• Wirt: Mouse