JAK3 Kinase Domain (human, recombinant)

Formulation: 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Purity: ≥90% estimated by SDS-PAGE

Formula Weight: 0

Shelf life (days): 180

Notes: JAK3 is a non-receptor tyrosine kinase that has roles in cytokine signaling and immune cell function.{72529,72530} It is composed of N-terminal FERM and SH2 domains, a regulatory JH2 pseudokinase domain, and a C-terminal kinase domain.{72530,19346} JAK3 is constitutively expressed in natural killer (NK) cells and thymocytes and expressed upon cell activation in T cells, B cells, and monocytes.{72531} Following cytokine binding to the IL-2 receptor (IL-2R), IL-4R, IL-7R, IL-9R, IL-15R, or IL-21R, JAK3 binds to the γc subunit of the receptor and induces heterodimerization of the receptor subunits and activation of STAT transcription factors.{72529,72530,72531} Through activation of these receptors, JAK3-mediated signaling is involved in T cell proliferation, differentiation, and survival, B cell differentiation and function, and macrophage activation, among other activities.{72529,72530} Loss-of-function mutations in JAK3 are associated with autosomal recessive severe combined immunodeficiency disease (SCID), while gain-of-function mutations are associated with immune dysregulation and blood cancers, including myeloproliferative neoplasms, T cell lymphomas and leukemias, NK lymphoma-leukemia, and acute lymphoblastic leukemia.{72530,72532,72533} Cayman's JAK3 Kinase Domain (human, recombinant) protein can be used for enzyme activity assays. This protein has a calculated molecular weight of 37 kDa.

• Artikelnummer: CAY42275-20
• Hersteller: Cayman Chemical
• Hersteller Artikelnummer: 42275-20
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Klonalität: Recombinant