JHDM1D (KDM7A) Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Contraindications: Green and blue dyes that absorb light in the AlphaScreen signalemission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygenquenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). Thepresence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence ofexcess biotin and iron in this medium. MEM, which lacks these components, does not affectAlphaScreen assays.

Description: The JHDM1D Homogeneous Assay Kit is designed to measure the activity of the JHDM1D for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Storage Stability: At least one year from date of receipt when stored as directed.

Supplied As: The JHDM1D Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JHDM1D for 100 enzyme reactions.

Warnings: Avoid multiple freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Horton J.R., et al. Nature Structural & Molecular Biology 2010; 17(1):38-43.

• Artikelnummer: BPS50420-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50420-1
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK