JHDM1D (KDM7A) Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Contraindications: Green and blue dyes that absorb light in the AlphaScreen signalemission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygenquenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). Thepresence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence ofexcess biotin and iron in this medium. MEM, which lacks these components, does not affectAlphaScreen assays.

Description: The JHDM1D Homogeneous Assay Kit is designed to measure activity of JHDM1D for screeningand profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC)domain-containing histone lysine demethylase that exhibits demethylation activity towarddimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit isa highly specific antibody that recognizes demethylated substrate. With this kit, only three simplesteps on a microtiter plate are required for demethylase activity detection. First, a samplecontaining JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beadsand primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Storage Stability: At least 6 months from date of receipt when stored as directed.

Supplied As: The JHDM1D Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JHDM1D for 384 enzyme reactions.

Uniprot: A0JNV9

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Horton J.R., et al. Nature Structural & Molecular Biology 2010; 17(1):38-43.

• Artikelnummer: BPS50420-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50420-2
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK