KEAP1, His-tag Recombinant

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Applications: Useful for studying protein binding and screening small molecular inhibitors

Assay Conditions: Binding of Keap1 to Nrf2 was measuredusing BPS Keap1:Nrf2 Assay Kit, Cat.#72020. Briefly, varying concentrations ofKeap1 were added to wells containingFITC-labeled Nrf2 peptide in assay bufferand incubated for 30 min. at room temp.Fluorescence was measured at ex 485nm,em 530 nm using a Bio-Tek fluorescentmicroplate reader.

Description: Human Keap1, also known as Kelch-likeECH-associated protein, INRF2, KIAA0132,and KLHL19, GenBank Accession No.NM_203500, a.a. 321-609 expressed in anE. coli cell expression system. MW = 32.6kDa

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mMimidazole, 3 mM DTT, 20% glycerol

Genbank: NM_203500

Storage Stability: At least 6 months at -80°C.

Tags: His-tag

Uniprot: Q14145

Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Dinkova-Kostova, A.T., et al., 2002. Proc. Nat. Acad. Sci. (USA). 99(18): 11908-11913.
2. Inoyama, D., et al. 2012. J. Biomol. Screening 17(4): 435-447.

• Artikelnummer: BPS70040-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 70040-2
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK