Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-186
Application: Useful for nucleotide exchange assay, KRAS(G12C) inhibitors screening, and selectivity profiling.
Assay Conditions: GppNHp loaded KRAS(G12C) was tested for binding to the RBD of Raf1. GppNHp loaded KRAS(G12C) was compared with GDP-loaded KRAS(G12C) (BPS Bioscience #100640) for RBD-RAF1 (BPS Bioscience #100519) binding by Bio-layer Interferometry (GATOR, Probe Life) and AlphaScreening (PerkinElmer).
Description: Human KRAS, also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-186 with G12C mutation and N-terminal His-tag, expressed in an E. coli expression system. MW=23 kDa. KRAS (G12C) His-Tag (BPS Bioscience #100413), is loaded with GppNHp (non-hydrolyzable GTP analog). Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT.
Genbank: NM_033360
Storage Stability: At least 6 months at -70°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: P01116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ostrem, J. M., et al., Nature. 2013;503:548-551.
2. Patricelli, M. P., et al., Cancer Discovery. 2016;6:316-329.
Deutsch
English
