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Encompassing Amino Acids: 2-185
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (SOS1 mediated) (>BPS Bioscience #78004) with various amounts of KRAS (G12V), His-Tag, GppNHp-Loaded.
Description: Recombinant human RAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The protein also contains mutation of interest G12V. The protein (BPS Bioscience #100480) was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column.
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT
Genbank: NM_004985
Purity: ≥90%
Storage Stability: At lease 6 months at -80°C
Supplied As: Aqueous buffer solution
Tags: N-terminal His-tag
Uniprot: P01116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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