Target Synonym: LATS1;WARTS;wts
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunogen: A synthetic peptide of human LATS1
Dilution: WB 1:500-1:2000;IHC 1:50-1:200
Calculated MW: 76kDa/126kDa
Observed MW: 150KDa
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