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Encompassing Amino Acids: 1-545(full length)
Description: Firefly Luciferase, also known as Luciferin 4-monooxygenase, Uniprot No. Q27757, a.a. 1-545(full length) with C-terminal His-tag, expressed in an E. coli expression system. MW=61 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 150 mM Imidazole and 20% glycerol.
Genbank: BAA93575.1
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Uniprot: Q27757
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. DE Wet, J.R., et al., Molec. Cell. Bio. 1987 Feb; 7(2):725-737.
2. Brasier, AR., et al., Euro. PMC. 1989 Oct; 7(10):1116-1122.