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MolBoolean Mouse / Rabbit
MolBoolean Mouse / Rabbit
Artikelnummer
ATLMolB00001
Verpackungseinheit
4.8 ml, approx. 120 assays (40 µl/assay)
Hersteller
Atlas Antibodies
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Product description: MolBoolean™ Mouse/Rabbit is a kit for in situ protein proximity analysis in tissue and cells. The MolBoolean assay utilizes a proprietary oligonucleotide setup that enables the simultaneous detection of both free and interacting (~40 nm proximity) fractions for two proteins of interest (protein A, protein B and interaction proteins AB).
MolBoolean™ Mouse/Rabbit should be performed with the user’s primary antibodies of choice (raised in mouse and rabbit). MolBoolean relies on anti-mouse and anti-rabbit secondary proximity probes and rolling circle amplification (RCA) as a mean to amplify signal. A series of molecular steps, performed in the MolBoolean assay, incorporates information into the amplified products indicating antibody target engagement of individual versus interacting proteins. This information is converted to fluorescent signals by the binding of detection reporters, allowing visualization on a conventional fluorescent microscope. An image analysis software is provided to segment and differentiate fluorescent signals, enabling the relative quantification of free versus interacting fractions for the two analyzed protein targets in the sample.
Included in the kit: Antibody proximty probes, oligonucleotides, enzymes, fluorescent detection reporters
Not included in the kit: Primary antibodies.
MolBoolean™ Mouse/Rabbit should be performed with the user’s primary antibodies of choice (raised in mouse and rabbit). MolBoolean relies on anti-mouse and anti-rabbit secondary proximity probes and rolling circle amplification (RCA) as a mean to amplify signal. A series of molecular steps, performed in the MolBoolean assay, incorporates information into the amplified products indicating antibody target engagement of individual versus interacting proteins. This information is converted to fluorescent signals by the binding of detection reporters, allowing visualization on a conventional fluorescent microscope. An image analysis software is provided to segment and differentiate fluorescent signals, enabling the relative quantification of free versus interacting fractions for the two analyzed protein targets in the sample.
Included in the kit: Antibody proximty probes, oligonucleotides, enzymes, fluorescent detection reporters
Not included in the kit: Primary antibodies.
Atlas Antibodies attaches great importance to sustainability. In the production process of their high quality antibodies, Atlas follows the highest and most rigorous ethical animal welfare standards. The products are produced within Europe, which reduces the supply chain length to a minimum and WoolCool (100% natural sheep wool) is used as an insulating material instead of Styrofoam for the transportation of refrigerated goods.
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Gernot Ensinger, M.Sc.
Laurent Haze
