Clone Number: MoBU-1 (85-2C8)
Immunogen: conjugate of bromodeoxyrudine and Helix pomatia hemocyanin (HPH)
Concentration: 100 ug/ml
Storage buffer: PBS with 0.02% sodium azide
Additional info: MoBU-1 is reactive with cells containing incorporated 5-bromodeoxyuridine (BrdU) showing a clear, nucleus confined, speckled pattern. BrdU is an analogue of thymidine and can be introduced to proliferating cells in vitro or in vivo, which in turn incorporate BrdU into the DNA during S phase, prior to cell division. Immunocytochemical staining with MoBU-1 will show the degree of proliferation, detecting nucleated cells which have incorporated BrdU in place of thymidine into their DNA.
References: Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988)/Arras et al. J Clin Invest. 101(1), 40-50 (1998)