Mouse anti-FoxP3 Purified, clone 3G3 (Monoclonal)

Clone Number: 3G3

Immunogen: Full-length His-tagged recombinant murine FoxP3

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.SpecificityThe antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.Application detailsWestern blotting: Recommended dilution: 2 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

• Artikelnummer: SANMON23042
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23042
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Monoclonal
• Methode: Western Blotting, Flow Cytometry
• Isotyp: IgG1 kappa
• Wirt: Mouse
• Konjugat: Unconjugated