Mouse anti-HLA-E Purified, clone MEM-E/07 (Monoclonal)

Clone Number: MEM-E/07

Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).SpecificityThe mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
Flow cytometry: Recommended dilution: 1-4 µg/ml

• Artikelnummer: SANMON23055
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23055
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (paraffin), Immunoprecipitation, Flow Cytometry
• Isotyp: IgG1
• Wirt: Mouse
• Konjugat: Unconjugated