Mouse anti-Hu CD38 Purified, clone HIT2 (Monoclonal)

Clone Number: HIT2

Immunogen: Human thymocytes in foetus

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.SpecificityThe mouse monoclonal antibody TU66, also known as Tü66, recognizes an extracellular epitope of CD39, a 78 kDa cell surface enzyme expressed by regulatory T cells, mantle zone B cells, activated T cells, NK cells, macrophages, dendritic cells, neurons, endothelial cells and platelets.Application detailsImmunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
Western blotting: Recommended dilution: 2 μg/ml; positive control: RAJI human cell line, non-reducing conditions.
Flow cytometry: Recommended dilution: 1-4 µg/ml

• Artikelnummer: SANMON23191
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23191
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western Blotting, Flow Cytometry
• Isotyp: IgG1
• Wirt: Mouse