Mouse anti-Hu CD49d Purified, clone 9F10 (Monoclonal)

Clone Number: 9F10

Immunogen: Unknown or proprietery to MONOSAN and/or its suppliers

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.SpecificityThe mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.Application detailsImmunohistochemistry: Recommended dilution: 5-10 μg/ml.

• Artikelnummer: SANMON23202
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23202
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Primate, Sheep (Ovine), Cat (Feline), Dog (Canine), Cow (Bovine), Horse (Equine)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Flow Cytometry
• Isotyp: IgG1 kappa
• Wirt: Mouse