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Mouse anti Human IgG4 (pFc subclass specific)
Mouse anti Human IgG4 (pFc subclass specific), Monoclonal, IgG1, Clone: NI 315 (HP 6206)
Artikelnummer
MAHu/IgG4/Mab
Verpackungseinheit
0,2 mg
Hersteller
Nordic-MUbio
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Clone: NI 315 (HP 6206)
Background: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Source: Highly purified monoclonal IgG4 isolated from human serum
Specificity: Monoclonal Mouse antibody to Human IgG4, subclass specific.In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.
Formulation: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Source: Highly purified monoclonal IgG4 isolated from human serum
Specificity: Monoclonal Mouse antibody to Human IgG4, subclass specific.In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.
Formulation: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | MAHu/IgG4/Mab |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | MAHu/IgG4/MAb |
| Green Labware | Nein |
| Verpackungseinheit | 0,2 mg |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Immunofluorescence, ELISA, Immunohistochemistry, Immunocytochemistry |
| Isotyp | IgG1 |
| Wirt | Mouse |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
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