Mouse anti Human p34 cdc-2 nuclear protein

Mouse anti Human p34 cdc-2 nuclear protein, Monoclonal, IgG2a, Clone: POH-1

Clone: POH-1

Background: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.

Positive Control: HeLa cell lysate

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

References: 1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-1762. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-278543. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784

UniProt: P06493

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1236M
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1236M
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Frog (Xenopus Laevis)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry
• Isotyp: IgG2a
• Wirt: Mouse
• Konjugat: Unconjugated