Mouse anti-Intra-acrosomal protein, clone Hs-8 (Monoclonal)

Clone Number: Hs-8

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Concentration: 1 mg/ml

Storage buffer: Tris buffered saline (TBS) solution with 15 mM sodium azide

Additional info: GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

• Artikelnummer: SANMON8016
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON8016
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Pig (Porcine)
• Klonalität: Monoclonal
• Methode: Western Blotting, Flow Cytometry, Immunocytochemistry
• Isotyp: IgM
• Wirt: Mouse