Mouse anti-Ki-67 Purified, clone Ki-67 (Monoclonal)

Clone Number: Ki-67

Immunogen: Nuclei of the Hodgkin lymphoma cell line L428

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.SpecificityThe antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.Application detailsFlow cytometry: Recommended dilution: 1-12 µg/ml. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.

• Artikelnummer: SANMON23276
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23276
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Cow (Bovine)
• Klonalität: Monoclonal
• Methode: Western Blotting, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Isotyp: IgG1
• Wirt: Mouse
• Konjugat: Unconjugated