Mouse anti-MART-1 (MELAN A), clone M2-9E3 (Monoclonal)

Clone Number: M2-9E3

Immunogen: Recombinant hMART-1 protein

Concentration: n/a

Format: Concentrate

Storage buffer: Bioreactor Concentrate with 0.05% Azide

Additional info: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive marker for angiomyolipoma’s. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Melanoma, normal skin. Staining Cytoplasmic

References: Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25./Marincola FM, et. al. J of Immunotherapy with Emphasis on Tumor Immunol, 1996, 19(3):192-205.

• Artikelnummer: SANMON10210
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON10210
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin)
• Isotyp: IgG2b kappa
• Wirt: Mouse