Mouse anti-OCT-3/4, clone C-10(Monoclonal)

Clone Number: C-10

Immunogen: Amino acids1-134 of Oct-3/4 of human origin

Concentration: n/a

Format: Purified

Storage buffer: Purified antibody in PBS with 0.2 % BSA and 15mM sodium azide

Additional info: Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Seminoma or embryonal carcinoma. Staining Nuclear

References: Drocourt, L., et al. 2002, J. Biol. Chem. 277: 25125-25132./Fong, Y.W., et al. 2011, Cell 147: 120-131./Wang, J., et al. 2011, Cancer Res. 71: 7238-7349.

• Artikelnummer: SANMON10219
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON10219
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin)
• Isotyp: IgG2b
• Wirt: Mouse