Mouse anti Yeast GAL-4 DNA Binding Domain

Mouse anti Yeast GAL-4 DNA Binding Domain, Monoclonal, IgG1, Clone: 8C-1

Clone: 8C-1

Background: Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

References: 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385.2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432.3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAZ150M
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: Z150M
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Yeast
• Klonalität: Monoclonal
• Methode: Immunoprecipitation, Western Blotting
• Isotyp: IgG1
• Wirt: Mouse
• Konjugat: Unconjugated