Mouse Interleukin-6 Recombinant

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Encompassing Amino Acids: 25-211

Amino Acid Sequence: FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT

Background: IL-6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL-6 cytokine family induce hepatic expression of acute phase proteins. IL-6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL-6 after stimulation. The IL-6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T-cells. The IL-6 receptor (CD126) is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids.

Biological Activity: The ED₅₀ determined by the dose-dependent proliferation of murine 7TD1 cells, was found to be < 0.01 ng/ml cells, corresponding to specific activity of 1 x 10⁸ U/mg.

Description: Recombinant murine IL-6 is a disulfide-linked homodimeric protein consisting of 188 amino acid residues, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse Interleukin-6 mature chain was expressed in E. coli.

Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Format: lyophilized protein

Formulation: Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

Genbank: P08505

Purity: ≥97% by SDS-PAGE and HPLC

Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Uniprot: P08505

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Am. J. Respir. Cell Mol. Biol., Oct 2009, 41: 385 - 396.
2. Ann Rheum Dis, Oct 2009, 68: 1580 - 1584.
3. Glycobiology, Oct 2009, 19: 1082 - 1093.

• Artikelnummer: BPS90197-B
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 90197-B
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK