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Nitrotyrosine ELISA Kit
Colorimetric detection of Nitrotyrosine
Artikelnummer
STRSKT-126-480
Verpackungseinheit
96 well x5
Hersteller
Stressmarq Biosciences
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Target: Nitrotyrosine
Product Type: ELISA Kits
Trademark: MOLECULAR SIGNATURE®
Detection Method: Colorimetric Assay
Assay Type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Utility: ELISA kit used to quantitate Nitrotyrosine in samples.
Precision: Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%.Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Sample Type(s): Plasma,Serum,Cell Lysates,Urine
Number of Samples: 38 samples in duplicate
Incubation Time: 1 hour
Sensitivity: 50 nM
Assay Range: 62.5 - 8000 nM
Scientific Background: Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present.Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Product Type: ELISA Kits
Trademark: MOLECULAR SIGNATURE®
Detection Method: Colorimetric Assay
Assay Type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Utility: ELISA kit used to quantitate Nitrotyrosine in samples.
Precision: Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%.Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Sample Type(s): Plasma,Serum,Cell Lysates,Urine
Number of Samples: 38 samples in duplicate
Incubation Time: 1 hour
Sensitivity: 50 nM
Assay Range: 62.5 - 8000 nM
Scientific Background: Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present.Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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