NKCC2 Antibody: RPE

Rabbit Anti-Rat NKCC2 Polyclonal

Target: NKCC2

Conjugate: RPE

Product Type: Polyclonal

Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).

Swiss-Prot: Q5PQV1

Purification: Protein A Purified

Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects ~160kDa.

Cellular Localization: Membrane

Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).

References: 1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971.2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• Artikelnummer: STRSPC-401D-RPE
• Hersteller: Stressmarq Biosciences
• Hersteller Artikelnummer: SPC-401D-RPE
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus)
• Klonalität: Polyclonal
• Methode: Western Blotting, Immunohistochemistry
• Human Gene ID: 25065
• Wirt: Rabbit