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Noggin Recombinant
Noggin Recombinant
Artikelnummer
BPS90256-2
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Applications: Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Background: Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
Biological Activity: Measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is <20 ng/ml in the presence of 30 ng/ml of human BMP-4.
Description: Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer). Source: human 293 cells. Endotoxin Level: <1 EU/µg. Biological Activity: measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4. Formulation: Lyophilized from a 0.2 µM filtered, aqueous solution. Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA.
Endotoxin Level: < 1EU per µg of Noggin as determined by the LAL method.
Format: Lyophilized solid
Formulation: Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA
Genbank: NM_005450
Reconstitution: Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles.
Tags: none
Uniprot: Q13253
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.
2. Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233- 2245.
3. Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
Applications: Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Background: Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
Biological Activity: Measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is <20 ng/ml in the presence of 30 ng/ml of human BMP-4.
Description: Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer). Source: human 293 cells. Endotoxin Level: <1 EU/µg. Biological Activity: measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4. Formulation: Lyophilized from a 0.2 µM filtered, aqueous solution. Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA.
Endotoxin Level: < 1EU per µg of Noggin as determined by the LAL method.
Format: Lyophilized solid
Formulation: Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA
Genbank: NM_005450
Reconstitution: Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles.
Tags: none
Uniprot: Q13253
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.
2. Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233- 2245.
3. Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
| Artikelnummer | BPS90256-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 90256-2 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
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| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
