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NPM1, His-Tag Recombinant
NPM1, His-Tag Recombinant
Artikelnummer
BPS102057-2
Verpackungseinheit
1 mg
Hersteller
BPS Bioscience
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Applications: Useful for SDS-PAGE.
Background: NPM1 (nucleophosmin), also known as nutramin or nucleolar phosphoprotein B23, is a protein that binds to nucleolar ribonucleoprotein structures and is involved in ribosome biogenesis, centrosome duplication, RNA helix destabilization, DNA repair, and tumor suppression. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Abnormalities in the NMP1 gene have been found in non-Hodgkin lymphoma, acute promyelocytic leukemia and acute myeloid leukemia (AML). In AML this protein was found to be mutated, lacking a folded C-terminal domain. The development of molecules able to either fold the protein to its native state or remove it from the cytosol may be beneficial in cancer therapy.
Description: Recombinant human NPM1 (nucleophosmin), encompassing amino acids 2-294(end). This construct contains an N-terminal His-tag (6xHis) followed by a TEV site. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 500 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 238 mM Imidazole
Genbank: NM_002520
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag
Uniprot: P06748
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Applications: Useful for SDS-PAGE.
Background: NPM1 (nucleophosmin), also known as nutramin or nucleolar phosphoprotein B23, is a protein that binds to nucleolar ribonucleoprotein structures and is involved in ribosome biogenesis, centrosome duplication, RNA helix destabilization, DNA repair, and tumor suppression. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Abnormalities in the NMP1 gene have been found in non-Hodgkin lymphoma, acute promyelocytic leukemia and acute myeloid leukemia (AML). In AML this protein was found to be mutated, lacking a folded C-terminal domain. The development of molecules able to either fold the protein to its native state or remove it from the cytosol may be beneficial in cancer therapy.
Description: Recombinant human NPM1 (nucleophosmin), encompassing amino acids 2-294(end). This construct contains an N-terminal His-tag (6xHis) followed by a TEV site. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 500 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 238 mM Imidazole
Genbank: NM_002520
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag
Uniprot: P06748
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS102057-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 102057-2 |
| Green Labware | Nein |
| Verpackungseinheit | 1 mg |
| Mengeneinheit | STK |
| Wirt | Escherichia Coli |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
