PARP1 Colorimetric Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Description: The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependentaddition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric ActivityAssay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylatedsubstrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plateis treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate toproduce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Brown, J.A., Marala, R.B. 2002. J. Pharmacol. Toxicol. Methods 47:137-41.

• Artikelnummer: BPS80580
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80580
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK