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PARP2 Chemiluminescent Assay Kit
PARP2 Chemiluminescent Assay Kit
Artikelnummer
BPS80552-2
Verpackungseinheit
384 rxns.
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput (HTS) applications.
Background: PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
Contraindications: The PARP2 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.
Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The PARP2 assay kit comes in a convenient 96-well or 384-well format, with purified PARP2 enzyme, histone mixture, activated DNA template, and PARP assay buffer for 100 or 400 enzyme reactions.
Uniprot: Q9UGN5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown, J.A., Marala, R.B., J. Pharmacol. Toxicol. Methods 2002;47:137-41.
Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput (HTS) applications.
Background: PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
Contraindications: The PARP2 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.
Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The PARP2 assay kit comes in a convenient 96-well or 384-well format, with purified PARP2 enzyme, histone mixture, activated DNA template, and PARP assay buffer for 100 or 400 enzyme reactions.
Uniprot: Q9UGN5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown, J.A., Marala, R.B., J. Pharmacol. Toxicol. Methods 2002;47:137-41.
| Artikelnummer | BPS80552-2 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80552-2 |
| Verpackungseinheit | 384 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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