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Encompassing Amino Acids: 25-167
Applications: Useful as a positive control for Western blot studies.
Description: Mouse secreted Programmed Cell Death 1 (PD-1)-Fc fusion protein, also known as CD279, PDCD1, and SLEB2, GenBank Accession No. NM_008798, a.a. 25-167, with a C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. MW = 19 kDa. This protein runs at an apparent higher molecular weight due to glycosylation.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_008798
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Avi-His-Tag
Biosafety Level: Not applicable (BSL-1)
References: 1. Agata, A., et al., Immunol. 1996 Oct;8(5):765-772.2. Freeman, G., et al., JEM. 2000 Oct; 192(7):1027-1034.