PD-L1 (CD274), Fc fusion (Human) HiP™ Recombinant

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Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.

Assay Conditions: PD-L1 binding to PD-1 was tested following protocol for BPS Cat. #72005. Briefly, wells were coated with 10 ng of PD-L1 in PD-1 assay buffer. Varying concentrations of biotinylated PD-1 was added and wells were incubated at room temp. for 2 hours before adding strep-HRP, followed by chemiluminescent substrate. Luminescence was read on a chemiluminescent plate reader.

Background: PD-L1 is involved in the costimulatory signal essential for T-cell proliferation and production of IL-10 and IFNgamma. Interaction with PD-1 inhibits T-cell proliferation and cytokine production, and down-regulates the immune response. PD

Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Genbank: NM_014143

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal Fc fusion (Human IgG1)

Uniprot: Q9NZQ7

Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.

Biosafety Level: Not applicable (BSL-1)

References: 1. Freeman, G.J., et al., J Exp Med. 2000 Oct 2;192(7):1027-34.
2. Dong, H., et al., Nat Med. 1999 Dec; 5(12):1365-9.Application Reference(s):
Imai, Y., et al. J Immunol. 2015 Jul 15;195(2):421-5. doi: 10.4049/jimmunol.1500448. Cutting Edge: PD-1 Regulates Imiquimod-Induced Psoriasiform Dermatitis through Inhibition of IL-17A Expression by Innate γδ-Low T Cells (2015)

• Artikelnummer: BPS71104-3
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71104-3
• Green Labware: Nein
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK