Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates.
Components: Eu-phospho-CREB (25 µL), Acc-phospho-CREB (100 µL), CREB control lysate (500 µL), 5X Lysis Buffer 1 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-ERK1/2 (T202/Y204).
Assay Target Class: Kinase.
SwissProt: P27361, P28482.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).