Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-GSK3β (S9) and Total GSK3β in cell lysates.
Components: Eu-phospho-GSK3b (20 µL), Acc-phospho-GSK3b (80 µL), Eu-Total GSK3b (5 µL), Acc-Total GSK3b (20 µL), GSK3b control lysate (500 µL), 5X Lysis Buffer 3 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-GSK3β (S9) and Total GSK3β.
Assay Target Class: Kinase.
SwissProt: P49841.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).