Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) and Total p70 S6K in cell lysates.
Components: Eu-phospho-P70S6 (20 µL), Acc-phospho-P70S6 (80 µL), Eu-Total P70S6 (5 µL), Acc-Total P70S6 (20 µL), P70S6 control lysate (500 µL), 5X Lysis Buffer 3 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-p70 S6K (T389) and Total p70 S6K .
Assay Target Class: Kinase.
SwissProt: P23443.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).