Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S240/S244) and Total Ribosomal Protein S6 in cell lysates.
Components: Eu-phospho-RPS6S240 (20 µL), Acc-phospho-RPS6S240 (80 µL), Eu-Total RPS6 (5 µL), Acc-Total RPS6 (20 µL), RPS6 control lysate (500 µL), 5X Lysis Buffer 4 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-Ribosomal Protein S6 (S240/244) and Total Ribosomal Protein S6 .
Assay Target Class: Kinase.
SwissProt: P62753.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).