Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) and Total STAT3 in cell lysates.
Components: Eu-phospho-STAT3 (20 µL), Acc-phospho-STAT3 (80 µL), Eu-Total STAT3 (5 µL), Acc-Total STAT3 (20 µL), STAT3 control lysate (500 µL), 5X Lysis Buffer 2 (5 mL), 10X TR-FRET Detection buffer (250 µL).
Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.
Assay Format: Half-area 96-well or low-volume 384-well plate.
Assay Target: Phospho-STAT3 (Y705) and Total STAT3.
Assay Target Class: Kinase.
SwissProt: P40763.
Detection method: TR-FRET.
Sample type: Cell lysates (adherent or suspension cells).