Application Note: WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 52
Positive Control: 2dpf zebrafish embryos
Form: Liquid
Buffer (with preservative): PBS, 20% Glycerol, 0.025% ProClin 300.
Concentration: 2.24 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.
Uniprot ID: Q90270
Antigen Species: Zebrafish
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Pou5f3 (Oct4). The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: POU domain, class 5, transcription factor 3