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PRMT3 Chemiluminescent Assay Kit
PRMT3 Chemiluminescent Assay Kit
Artikelnummer
BPS52005L
Verpackungseinheit
96 rxns.
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highlyspecific antibody that recognizes methylated substrate. With this kit, only three simplesteps on a microtiter plate are required for methyltransferase detection. First,Sadenosylmethionine is incubated with a sample containing assay buffer andmethyltransferase enzyme for one hour. Next, primary antibody is added. Finally, theplate is treated with an HRP-labeled secondary antibody followed by addition of the HRPsubstrate to produce chemiluminescence that can then be measured using achemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with specific substrate, reagents, and purified PRMT3 enzyme for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highlyspecific antibody that recognizes methylated substrate. With this kit, only three simplesteps on a microtiter plate are required for methyltransferase detection. First,Sadenosylmethionine is incubated with a sample containing assay buffer andmethyltransferase enzyme for one hour. Next, primary antibody is added. Finally, theplate is treated with an HRP-labeled secondary antibody followed by addition of the HRPsubstrate to produce chemiluminescence that can then be measured using achemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with specific substrate, reagents, and purified PRMT3 enzyme for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
| Artikelnummer | BPS52005L |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 52005L |
| Green Labware | Nein |
| Verpackungseinheit | 96 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
