Clone: Polyclonal
Background: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal bovine serum and purified bovine albumin. One characteristic precipitin line is obtained against pooled normal bovine serum using different antigen/antibody concentration ratio’s. Precipitin lines against normal bovine serum and purified bovine albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in bovine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified albumin isolated from pooled bovine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Horseradish peroxidase–conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine albumin.
Formulation: Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.