Rabbit anti Bovine beta lactoglobulin

Rabbit anti Bovine beta lactoglobulin, Polyclonal, Clone: Polyclonal

Clone: Polyclonal

Background: The defined antibody specificity is directed to beta-lactoglobulin as tested against bovine milk. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) no reaction has been obtained with bovine serum. As precipitating antiserum to identify or measure bovine beta-lactoglobulin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Source: Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue. It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point. The molecular weight of the monomeric subunit is near 17,000. It is very sensitive to denaturation. Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation. Three genetic variants A, B and C have been identified. They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions. No immunological differences was found between the variants. Beta-lactoglobulin can be purified in crystallized form. Allergic reactions of infants to bovine milk proteins are well known. The actual process by which such reactions develop and their impact on the infant’s health have not been unambiguously established. Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Specificity: Precipitating polyclonal Rabbit antiSerum to Bovine beta-lactoglobulin.

Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

• Artikelnummer: RAB/BLgl
• Hersteller: Nordic-MUbio
• Hersteller Artikelnummer: RAB/BLgl
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Cow (Bovine)
• Klonalität: Polyclonal
• Methode: Immunoprecipitation
• Wirt: Rabbit
• Konjugat: Unconjugated