Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to the Fc part of the IgE molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum, a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgE. No precipitation reaction is obtained with purified IgG, IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) to identify the presence of IgE in human serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Source: Highly purified homogenous IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Precipitating polyclonal Rabbit antiSerum to Human IgE, Fc specific.
Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.