Rabbit anti Human Toll-like receptor 3 (TLR3) (NT)

Rabbit anti Human Toll-like receptor 3 (TLR3) (NT), Polyclonal, IgG

Background: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection (4). Recently it has been shown to recognize viruses such as Influenza A (5) and West Nile Virus and can mediate entry of at least West Nile Virus (6).

Positive Control: Located in the membrane and expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors.7.

Immunogen: Rabbit polyclonal TLR3 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR3.

Purification Method: Antigen Immunoaffiinity Purification.

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide.

References: 1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
2) Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
3) McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82.
4) Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001; 413:732-8.
5) Guillot L, Le Goffic R, Bloch S, et al. Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A virus. J. Biol. Chem. 2005; 280:5571-80.
6) Wang T, Town T, Alexopoulou L, et al. Toll-like receptor 3 mediates West Nile virus entry into the brain causing lethal encephalitis. Nat. Med. 2004; 10:1366-73.
7) UniProtKB/Swiss-Prot entry O15455, http://www.expasy.org/uniprot/O15455, Accessed February 2, 2007.

UniProt: O15455.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1811P
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1811P
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Polyclonal
• Methode: Immunohistochemistry (paraffin), Western Blotting
• Isotyp: IgG
• Wirt: Rabbit