Rabbit anti Human Toll-like receptor 6 (TLR6) (NT)

Rabbit anti Human Toll-like receptor 6 (TLR6) (NT), Polyclonal, IgG

Background: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR6 was first identified as a close homolog of TLR1, sharing 69% sequence identify (4). Like TLR1, TLR6 can form heterodimers with TLR2, and these TLR6:TLR2 dimers coordinate macrophage activation by Gram-positive bacteria and the yeast cell wall particle zymosan (5). Activation of these complexes not only initiates pro-inflammatory cascades, but also can lead to apoptotic responses (6).

Positive Control: Positive Control Jurkat cell Lysate. Located in the membrane, TLR6 is detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.7.

Immunogen: Rabbit polyclonal TLR6 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR6.

Purification Method: Antigen Immunoaffiinity Purification.

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide.

References: 1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
2) Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
3) McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol. Imm. 2004; 41:577-82.
4.) Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding Toll-like receptor family. Gene 1999; 231:59-65.
5) Ozinsky A, Underhill DM, Fontenot JD, et al. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc. Natl. Acad. Sci. USA 2000; 97:13766-71.
6) Into T, Kiura K, Yasuda M, et al. Stimulation of human Tolllike receptor (TLR) 2 and TLR6 with membrane lipoproteins of Mycoplasma fermentans induces apoptotic cell death after NF-kappa B activation. Cell Microbiol. 2004; 6:187-99. (WD0506)
7) UniProtKB/Swiss-Prot entry Q9Y2C9, http://www.expasy.org/uniprot/Q9Y2C9, Accessed 2/9/2007.

UniProt: Q9Y2C9.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1816P
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1816P
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Polyclonal
• Methode: Western Blotting, Immunocytochemistry
• Isotyp: IgG
• Wirt: Rabbit