Rabbit anti Human UEV1A

Rabbit anti Human UEV1A, Polyclonal, IgG

Background: Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).

Positive Control: Postive Control: Jurkat Cell Lysate.
UE1A is one of seven named isoforms of UEV1. UEV1A is located in the nucleus, while iosforms 3 and 5 are found in the membrane7.

Immunogen: Rabbit polyclonal UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.

Purification Method: Antigen Immunoaffiinity Purification.

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide.

References: 1) Sancho E, Vila MR, Sanchez-Pulido L, et al. Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells.Mol. Cell Biol. 1998; 18:576-89.
2) Akira S and Takeda K. Toll-like receptor Signalling.Nat. Rev. Immunol. 2004; 4:499-511
3) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.Mol. Interv. 2003; 3:466-77
4) Deng L, Wang C, Spencer E, et al. Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 2000; 103:351-61.
5) Wang C, Deng L, Hong M, et al. TAK1 is a ubiquitindependent kinase of MKK and IKK. Nature 2001; 412:346-51.
6) Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11.
7) Alternative product of entry: Q13404, http://www.expasy.org/uniprot/Q13404-2, Accessed Febraury 27, 2007.

UniProt: Q13404-2.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1829P
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1829P
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus)
• Klonalität: Polyclonal
• Methode: Western Blotting
• Isotyp: IgG
• Wirt: Rabbit