English
Rabbit anti Mouse IgG Fab, conjugated with FITC
Rabbit anti Mouse IgG Fab, conjugated with FITC, Polyclonal, Clone: Polyclonal
Artikelnummer
RAM/Fab/FITC
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio
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Clone: Polyclonal
Background: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotypespecific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified Fab of normal mouse IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse Fab of IgG.
Formulation: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Background: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotypespecific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified Fab of normal mouse IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse Fab of IgG.
Formulation: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
| Artikelnummer | RAM/Fab/FITC |
|---|---|
| Hersteller | Nordic-MUbio |
| Hersteller Artikelnummer | RAM/Fab/FITC |
| Green Labware | Nein |
| Verpackungseinheit | 1 ml |
| Mengeneinheit | STK |
| Reaktivität | Mouse (Murine) |
| Klonalität | Polyclonal |
| Methode | Immunofluorescence, Immunohistochemistry (frozen), ELISA, Immunocytochemistry, Immunofluorescence (indirect) |
| Wirt | Rabbit |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
